LabDialysisChat - Choice of dialysis tubing for DNA purification

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Choice of dialysis tubing for DNA purification

amidon7

2001-08-03

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I would like to purify a ~12kb DNA fragment from a restriction digest, for pronuclear injection of one cell mouse embryos. Which dialysis tubing would you recommend for this purpose? I would prefer one that is compatible with electro-elution of this DNA fragment from an agarose gel slice.
Thanks very much for your help

Michael

	Re: Choice of dialysis tubing for DNA purification

dave

2001-08-03

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A good place to start is Spectra/Por 4 (when in doubt use Spectra/Pos 4).

While your DNA fragment has a very high molecular weight, it is a linear and not globular molecule. This allows it to escape from tubing with a smaller MWCO than the fragment weight.

Any of the cellulosic membranes would be usable in your electroelution apparatus. If you feel that the MWCO of the Spectra/Por 4 is to low, you can use any of the Spectra/Por 6 or 7 membranes instead.

Thanks for visiting.

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Choice of dialysis tubing for DNA purification by amidon7 on 2001-08-03

Re: Choice of dialysis tubing for DNA purification by dave on 2001-08-03

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