Spectra/Por, the only name in laboratory dialysis.

LabDialysisChat.com:   The Answers for All of Your Laboratory Dialysis Questions.


Close MessageUserDate(yyyy-mm-dd)
Previous Subject
Next Subject		 Which dialyse membrane for my application?bizet2001-04-17Click here to register.
Hello,
I want to purify bacterial DNA after enzymatic lysis of a suspension of bacteria. I want to concentrate DNA who have a lenght about 50 Kb and eliminate proteins and peptides that appear during the lysis. Which membrane could you recommend me.
Thank you
Karine
 
Re: Which dialyse membrane for my application?
dave2001-04-18Click here to register.
Karine:

I would recommend either a 50,000 MWCO Spectra/Por 6 or a 100,000 MWCO Spectra/Por CE membrane to start. The DNA strand will be large in terms of molecular weight (more than 5 million daltons for 50,000 base-pairs), but it will be stringy and not globular. Since it's stringy it will be more likely to go through a smaller pore than a similarly weighted globular protein. The 50k/100k MWCO should let most of the proteins and peptides out but still retain most of the DNA.

Let me know how it works out.

Thanks for visiting.
  • To ask question or answer a question, you need to register here.
  • You can browse by clicking on a message subject to see the complete message.
  • If you have previously registered, you can log in below.

Message (click on a subject to view the messages)
Showing Oldest Message
Click for newer messages
NewestFirst LastNewer
Search for messages that contain  

You must register before asking a question or providing an answer.
You can click here to register. If you have already registered you can log in below.
(If you check the Use Cookies box then your computer will remember your log in name.)
User Name:
Password:


Click here if you previously registered and remember your user name but not your password.
Email us at ldc@lplc.com if you are having other problems.


Back to Top
All the Perl that's Practical to Extract and Report