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Message | User | Date(yyyy-mm-dd) |
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A question about the move of water? | taiga | 2001-05-17 | Click here to register. |
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Thank you for your answers. If I understand the principle, I'll a move of water through a tubing from inside tubing to outside, but what is happened for the SeO3Na (170 g/mol), it will pass outside? I'd like to have it in dialysat. I don't know the weight molecular of peptides, but I suppose that are enough big to stay in tubing. I'LL make analysis of dialisat to know if it there amino acid. Form size of membrane tubing, if I take 100D it's too small to make to separate Se03 Na2 from free-peptide and Se peptide.
It 's to be sur to choose the best membrane tubing.
Thank you.
Armelle | | |
| dave | 2001-05-17 | Click here to register. |
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 | The thing with water is, if you have a high salt concentration inside the tubing and a low salt concentration outside, water will migrate into the dialysis bag and may cause it to burst. You want the ionic strength inside and outside the bag to be as nearly equal as possible. You can use Dextrans or cross-linked starch outside the bag offset this effect (but not too much, of coarse, or else water will move out of the bag).
The MWCOs for dialysis tubing are generally for organic molecules. Selenium oxide will be smaller than an organic of the same molecular weight. It will probably go through the 100 MWCO tubing and will definitely go through the 500. |
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