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Message | User | Date(yyyy-mm-dd) |
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Dialysis vs. Diafiltration | rhansen | 2010-02-22 | Click here to register. |
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| We require a dialysis step in our aseptic processing of a fluid containing deionized water, protein and metabolic impurities. The fluid volume is 100 mL to 500 mL per run and we require a 10,000 MWCO, recovering the retentate. The process should be aseptic. Should we focus on dialysis or diafiltration? Membranes should be non-protein binding. Prefer disposables. Thank you. | | |
| wtamashiro | 2010-02-24 | Click here to register. |
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 | Both diafiltration and dialysis can be done using a 10 kD membrane. However, given the choice, I usually recommend diafiltration over dialysis when possible .As long as the molecular species you are trying to recover can withstand the mild shear stresses of Tangential Flow Filtration, diafiltration will always be the most efficient and quickest means of separation. However, if your sample is delicate, i.e easily affected or altered in a deleterious manner, than dialysis would be the more preferred method of separation, gentle but slow. Dialysis also tends to be the more economic approach. However, the costs saved by the quicker processing with TFF may offset this difference. In order to better determine the ideal approach for your particular application, I recommend that you contact our Technical service at 800-634-3300. There are many factors to be weighed when deciding which approach is better.
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