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Next Subject		 Lab dialysis with Micelles and Polymer Nanoparticlesbvslaughter2009-11-02Click here to register.
I have 3 questions:

1. Is there a guide regarding how concentrated a solution for dialysis should be? Is there any real problem with dialysis near the solubility limit (inside the bag) of a sample?

2. Can you recommend a way to correlate micelle size with surfactants to molecular weight? I am trying to remove surfactants that have an aggregation number of about 25, so the molecular weight is around 10,000. But, the particles are probably much larger than proteins of a similar molecular weight. I am planning on trying MWCO 100,000 to remove them from polymer particles that have an average size of about 150 nm diameter. Is there a way to find out the pore size for your dialysis membranes as a linear dimension instead of MWCO?

3. If I do use a large MWCO membrane, say 100 kD, and I want to equalize osmotic pressure, can you recommend what I would want to dissolve into the dialysate to accomplish this?

Thanks,

Brandon

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