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Next Subject		 For Anion Chromatographypotatoboy2011-03-28Click here to register.
I want to dialyze some samples of extracted plant tissue for anion analysis. I'm going to do ion chromatography using a Metrohm system. This company has a fancy in-line dialysis system that uses a polyamide membrane to separate the analytes (ions) from the junk (all other organic molecules). I don't have a fancy inline system so I need to do this dialysis old school. Do you know what would be equivalent to the dialysis membrane (polyamide) they used?
http://misp.metrohm.com/methods/dialysis/IC_systems.html

Thanks
 
Re: For Anion Chromatography
stevele2011-03-28Click here to register.
Dear Potatoboy, I believe their membrane is a .2um membrane. Our largest lab dialysis membrane is 1000kD, which is still smaller than .2um should work.
 
Re: For Anion Chromatography
potatoboy2011-03-30Click here to register.
Hi Stevele,

Thanks. You are correct that they us a 0.2 um membrane. I am surprised because I've seen claims that after 5 min of dialysis with this pore size macro-molecules do not pass into the acceptor solution but analytes do. But I'm pretty sure that all macro molecules are smaller than 0.2 um.

Anyway for my application I only want the ions, so I should just be able to us the smallest pore size, right? You have 100-500 D MWCO
 
Re: For Anion Chromatography
stevele2011-04-06Click here to register.
Yes, using the smallest MWCO would retain anything bigger than that size and still pass your ions.

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